Solution A
Internal Solution-ID: S4409
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 3.00 | g | 2.985 | 21.016 | |
| KH2PO4 | 0.20 | g | 0.199 | 1.462 | |
| NH4Cl | 0.30 | g | 0.299 | 5.581 | |
| NaCl | 7.00 | g | 6.965 | 119.185 | |
| KCl | 0.50 | g | 0.498 | 6.674 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.149 | 1.015 | |
|
Yeast extract
(BD-Difco) |
0.10 | g | 0.1 | - | |
|
Trace element solution SL12
(see below) |
1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| HEPES | 2.38 | g | 2.368 | 9.937 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 960.00 | ml | - | - | |
| 1 Mix ingredients, adjust pH to 7.5 and autoclave under a N2 atmosphere. After cooling, add the following stock solutions (filter-sterilized or *autoclaved) to the solution: | |||||
|
MgCl2 x 6 H2O
(*, 15%) |
10.00 | ml | - | - | |
|
NaHCO3
(8%) |
30.00 | ml | - | - | |
| Vitamin solution | 1.00 | ml | - | - | |
| Thiamine solution | 1.00 | ml | - | - | |
| Vitamin B12 solution 0.05 g/L | 1.00 | ml | - | - | |
Media with this solution
| J576 | DESULFATIBACILLUS OLEFINIVORANS MEDIUM |