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Solution A

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Internal Solution-ID: S4415

Equipment needed: Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.3 g 0.297 2.181
K2HPO4 0.3 g 0.297 1.704
NH4Cl 1.0 g 0.989 18.492
NaCl 1.0 g 0.989 16.925
KCl 0.1 g 0.099 1.327
Yeast extract 1.0 g 0.989 -
Trace minerals 10.0 ml - -
Distilled water 1000.0 ml - -
1 To complete medium, aseptically add the following solutions to the medium (per 10 ml):
Solution B 0.1 ml - -
Sodium acetate
(filter-sterilized, 1.0 M) 		0.2 ml - -
NaHCO3
(filter-sterilized, 5%) 		0.4 ml - -
2 Mix ingredients and adjust pH to 7.2. Distribute the solution into tubes (e.g., 10 ml in the Hungate tubes) under a N2-CO2 gas mixture (4:1, v/v), seal with butyl rubber stoppers and autoclave.
J579 BACILLUS AA MEDIUM