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Trace element solution

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Internal Solution-ID: S4562

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.800 g 12.8 66.967
FeCl2 x 4 H2O 0.200 g 0.2 1.006
MnCl2 x 4 H2O 0.100 g 0.1 -
CoCl2 x 6 H2O 0.170 g 0.17 1.309
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnCl2 0.100 g 0.1 0.734
CuCl2 x 2 H2O 0.020 g 0.02 0.117
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.026 g 0.026 0.201
NaCl 1.000 g 1 17.112
Na2SeO3 0.020 g 0.02 -
Distilled water 1000.000 ml - -
1 Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH 6.5 with KOH. Then add the remaining salts and adjust volume to 1000 ml.
J671 THERMOANAEROBIUM MEDIUM