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Solution A

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Internal Solution-ID: S4583

Equipment needed: Autoclave Hungate tubes Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.000 g 0.998 18.658
NaNO3 2.550 g 2.545 29.942
KH2PO4 0.080 g 0.08 0.587
MgCl2 x 6 H2O 4.500 g 4.491 22.09
CaCl2 x 2 H2O 0.375 g 0.374 2.546
NaCl 17.000 g 16.966 290.316
FeCl2 solution 1.000 ml - -
Trace element solution 1.000 ml - -
NaHCO3 3.600 g 3.593 42.768
Distilled water 1000.000 ml - -
1 To complete the medium, anaerobically and aseptically add the following solutions (autoclaved under a N2 atmosphere) to the medium (per 10 ml):
Yeast extract
(10%) 		0.100 ml - -
Sodium acetate
(1 M) 		0.100 ml - -
2 Mix components and adjust pH to 7.0. Dispense the solution into culture vessels (e.g., 10 ml in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas stream, seal with butyl rubber stoppers and autoclave.
3 Comment: Sodium nitrate can be substituted by ferric citrate (20 mM).
J683 FRR MEDIUM