Solution A
Internal Solution-ID: S4591
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 10.0 | g | 9.99 | 170.945 | |
| Yeast extract | 2.0 | g | 1.998 | - | |
| Trypticase peptone | 2.0 | g | 1.998 | - | |
| NH4Cl | 1.0 | g | 0.999 | 18.676 | |
| MgCl2 x 6 H2O | 1.0 | g | 0.999 | 4.914 | |
| KCl | 0.5 | g | 0.5 | 6.7 | |
| CaCl2 x 2 H2O | 0.4 | g | 0.4 | 2.718 | |
| K2HPO4 | 0.4 | g | 0.4 | 2.294 | |
| Resazurin | 0.5 | mg | 5.0e-4 | 0.002 | |
| Trace minerals | 10.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
| NaHCO3 | 4.0 | g | 3.996 | 47.568 | |
| Distilled water | 920.0 | ml | - | - | |
| 1 Mix components except NaHCO3, bring to a boil for several seconds and cool down under a stream of N2-CO2 (80:20). Add NaHCO3, mix thoroughly and distribute the medium into culture vessels under the same gas mixture. Seal with butyl rubber stoppers and autoclave. Finally, add per liter the following sterile solutions (autoclaved or *filter-sterilized): | |||||
|
Methanol solution (see below)
(*) |
50.0 | ml | - | - | |
|
Trace vitamins (see Medium No. 197)
(*) |
10.0 | ml | - | - | |
|
L-Cysteine HCl x H2O
(5%) |
5.0 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
5.0 | ml | - | - | |
Media with this solution
| J687 | MB MEDIUM WITH METHANOL |