Main sol. J695
Internal Solution-ID: S4604
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 25.00 | g | 23.81 | 407.418 | |
| NaNO3 | 1.00 | g | 0.952 | 11.205 | |
| NH4Cl | 0.33 | g | 0.314 | 5.876 | |
| KCl | 0.33 | g | 0.314 | 4.216 | |
| CaCl2 x 2 H2O | 0.33 | g | 0.314 | 2.138 | |
| MgCl2 x 6 H2O | 0.33 | g | 0.314 | 1.546 | |
| MES | 1.95 | g | 1.857 | 9.512 | |
| Resazurin | 1.00 | mg | 9.5e-4 | 0.004 | |
| Trace minerals | 10.00 | ml | - | - | |
|
Sulfur
(powder) |
12.00 | g | 11.429 | 356.363 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components except sulfur, adjust pH to 6.0 and autoclave under a N2 atmosphere. Distribute the medium into culture vessels (e.g., 20 ml in 100 ml serum bottles) containing appropriate amount of sulfur under a H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized) into the medium (per liter): | |||||
|
Trace vitamins (see Medium No. 197)
(*) |
10.00 | ml | - | - | |
|
KH2PO4
(2%) |
20.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
| 2 Pressurize the inoculated culture vessel to 200 kPa H2-CO2 (4:1, v/v). | |||||
Media with this solution
| J695 | NAUTILIA ABYSSI MEDIUM |