Main sol. J696
Internal Solution-ID: S4605
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.00 | g | 19.608 | 335.521 | |
| MgCl2 x 6 H2O | 3.00 | g | 2.941 | 14.467 | |
| Na2SO4 | 3.00 | g | 2.941 | 20.707 | |
| KCl | 0.50 | g | 0.49 | 6.575 | |
| NH4Cl | 0.30 | g | 0.294 | 5.499 | |
| KH2PO4 | 0.10 | g | 0.098 | 0.72 | |
| PIPES | 1.73 | g | 1.696 | 5.609 | |
| CaCl2 x 2 H2O | 0.11 | g | 0.108 | 0.734 | |
| Trace minerals | 10.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
| Na2S x 9 H2O | 0.30 | g | 0.294 | 1.225 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components except Na2S x 9H2O and adjust pH to 6.7. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a gas stream of H2-CO2 (4:1, v/v), seal with butyl rubber stoppers and autoclave. Separately autoclave 3% Na2S x 9H2O solution under a N2 gas atmosphere. Prior to inoculation, reduce the medium by the addition of the Na2S x 9H2O solution. Pressurized the inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v). | |||||
Media with this solution
| J696 | THERMODESULFATATOR ATLANTICUS MEDIUM |