Main sol. J698
Internal Solution-ID: S4607
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 0.5 | g | 0.499 | 2.025 | |
| (NH4)2SO4 | 0.4 | g | 0.399 | 3.021 | |
| K2HPO4 | 0.2 | g | 0.2 | 1.146 | |
| KCl | 0.1 | g | 0.1 | 1.339 | |
| FeSO4 x 7 H2O | 10.0 | mg | 0.01 | 0.036 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 2.0 with H2SO4. After autoclaving, add the following solution (autoclaved): | |||||
|
Yeast extract
(10%, BD-Difco) |
2.5 | ml | - | - | |
| 2 Comment: For autotrophic growth on ferrous sulfate, prepare the medium without yeast extract, add 13.9 g/L (final) FeSO4 x 7H2O and adjust pH to 1.7 with H2SO4. | |||||
Media with this solution
| J698 | ACIDIMICROBIUM MEDIUM |