Main sol. J707
Internal Solution-ID: S4624
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 1.00 | g | 0.966 | - | |
| Na2SO4 | 7.10 | g | 6.86 | 48.296 | |
| L-Sodium lactate | 6.95 | g | 6.715 | 59.923 | |
| MgSO4 x 7 H2O | 1.97 | g | 1.903 | 7.722 | |
| NH4Cl | 1.08 | g | 1.043 | 19.508 | |
| K2HPO4 | 0.38 | g | 0.367 | 2.108 | |
| CaCl2 x 2 H2O | 0.09 | g | 0.087 | 0.591 | |
| Trace vitamins | 2.00 | ml | - | - | |
|
Coenzyme M
(0.01%) |
0.20 | ml | - | - | |
| Vitamin B12 solution 0.05 g/L | 0.10 | ml | - | - | |
| Trace element solution | 12.50 | ml | - | - | |
| HEPES | 5.96 | g | 5.758 | 24.164 | |
| Resazurin | 1.00 | mg | 9.7e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Autoclave under a N2 gas atmosphere. After cooling, add the following sterile solutions (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 7%) |
10.00 | ml | - | - | |
|
L-Cysteine HCl x H2O
(2.5%) |
10.00 | ml | - | - | |
| 2 Adjust pH to 7.2. | |||||
Media with this solution
| J707 | LACTATE SULFATE MEDIUM |