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Trace element solution

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Internal Solution-ID: S4007

This solution is used in 6 media and was first defined in medium J301.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.80 g - -
FeSO4 x 7 H2O 0.42 g - -
H3BO3 0.01 g - -
MnCl2 x 4 H2O 0.10 g - -
CoCl2 x 6 H2O 0.17 g - -
CuCl2 x 2 H2O 0.02 g - -
ZnSO4 x 7 H2O 0.21 g - -
Na2MoO4 x 2 H2O 0.01 g - -
1 Dissolve nitrilotriacetic acid in 800 ml distilled water and adjust pH to 6.5 with NaOH. Then proceed to add the minerals and bring volume to 1.0 L.
J301 THERMODESULFOBACTERIUM HYDROGENOPHILUM MEDIUM
J403 ACETOBACTERIUM SUBMARINUS MEDIUM
J447 METHANOBACTERIUM ADHERENS MEDIUM
J707 LACTATE SULFATE MEDIUM
J1108 TEPIDIBACILLUS B5 MEDIUM
J1223 WIDDEL FRESHWATER MEDIUM WITH LACTATE