Main sol. J717
Internal Solution-ID: S4640
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.75 | g | 0.737 | 5.414 | |
| K2HPO4 | 1.50 | g | 1.473 | 8.46 | |
| NaCl | 0.90 | g | 0.884 | 15.128 | |
| MgCl2 x 6 H2O | 0.20 | g | 0.196 | 0.966 | |
| NH4Cl | 0.90 | g | 0.884 | 16.528 | |
| Trace mineral solution | 9.00 | ml | - | - | |
| Trace vitamins | 5.00 | ml | - | - | |
|
FeSO4 x 7 H2O
(10%) |
5.00 | �l | - | - | |
|
Tryptone
(BD-Difco) |
2.00 | g | 1.965 | 27.64 | |
| Yeast extract | 2.00 | g | 1.965 | - | |
|
Casamino acids
(BD-Difco) |
2.00 | g | 1.965 | - | |
| Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly. Bring to a boil, cool down and distribute into culture vessels under a N2 gas stream. Seal with butyl rubber stoppers and autoclave. Prior to inoculation, add the following solution (autoclaved under a N2 atmosphere) into the medium (per liter): | |||||
|
Na2S x 9 H2O
(5%) |
4.00 | ml | - | - | |
Media with this solution
| J717 | TYE MEDIUM FOR STRAIN AeB |