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Solution A

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Internal Solution-ID: S4659

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.50 g 0.579 10.832
MgSO4 x 7 H2O 0.50 g 0.579 2.351
CaCl2 x 2 H2O 0.25 g 0.29 1.97
NaCl 2.25 g 2.607 44.613
Trace vitamins 10.00 ml - -
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Se/W solution 1.00 ml - -
Betaine 6.70 g 7.764 66.271
Yeast extract 2.00 g 2.318 -
Casitone
(BD-Difco) 		2.00 g 2.318 -
Resazurin 1.00 mg 0.001 0.005
Distilled water 850.00 ml - -
1 Autoclave under a N2 gas atmosphere.
J731 SPOROMUSA MEDIUM