Main sol. J737
Internal Solution-ID: S4668
Equipment needed: Autoclave
Anaerobic workbench
Filter
| 1 Mix components under a N2-CO2 (80:20, v/v) gas mixture and autoclave under the same gas mixture in bottles sealed with butyl rubber stoppers. After cooling, aseptically and anaerobically add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized) to the medium: | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.85 | g | 0.847 | 15.843 | |
| NaCl | 0.85 | g | 0.847 | 14.501 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.678 | |
| MgCl2 x 6 H2O | 0.28 | g | 0.279 | 1.373 | |
| Trace metal solution | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Yeast extract | 0.20 | g | 0.199 | - | |
| Resazurin | 0.50 | mg | 5.0e-4 | 0.002 | |
| Distilled water | 830.00 | ml | - | - | |
| Phosphate solution | 25.00 | ml | - | - | |
|
Trace vitamins (see Medium No. 197)
(*) |
1.00 | ml | - | - | |
|
NaHCO3
(*, 8%) |
40.00 | ml | - | - | |
|
L-Sodium lactate
(*, 0.1 M) |
100.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
5.00 | ml | - | - | |
Media with this solution
| J737 | BM FOR SYNTROPHICUS SCHINKII |