Main sol. J751
Internal Solution-ID: S4689
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 25.00 | g | 24.728 | 423.135 | |
| NaNO3 | 1.00 | g | 0.989 | 11.637 | |
| NH4Cl | 0.33 | g | 0.326 | 6.102 | |
| KCl | 0.33 | g | 0.326 | 4.378 | |
| CaCl2 x 2 H2O | 0.33 | g | 0.326 | 2.22 | |
| MgCl2 x 6 H2O | 0.33 | g | 0.326 | 1.606 | |
| HEPES | 2.38 | g | 2.354 | 9.878 | |
| Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
| Trace minerals | 10.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
|
Sulfur
(powder) |
12.00 | g | 11.869 | 370.11 | |
| Distilled water | 900.00 | ml | - | - | |
| 1 Mix components except sulfur, adjust pH to 6.8 and autoclave under a N2-CO2 (4:1, v/v) atmosphere. Distribute medium into culture vessels (e.g., 18 ml in 100 ml serum bottles) containing appropriate amount of sulfur under a N2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized) to the above described basal medium (900 ml): | |||||
|
Sodium formate
(15%) |
20.00 | ml | - | - | |
|
NaHCO3
(*, 5%) |
50.00 | ml | - | - | |
|
Trace vitamins (see Medium No. 197)
(*) |
10.00 | ml | - | - | |
|
Na2S x 9 H2O
(3%) |
20.00 | ml | - | - | |
| 2 Readjust pH of the medium to 6.8, if necessary. | |||||
Media with this solution
| J751 | MODIFIED NAUTILIA LITHOTROPHICA MEDIUM |