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JCM 751 . MODIFIED NAUTILIA LITHOTROPHICA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J751 #

ml
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 25.00 g 24.728 423.135
NaNO3 1.00 g 0.989 11.637
NH4Cl 0.33 g 0.326 6.102
KCl 0.33 g 0.326 4.378
CaCl2 x 2 H2O 0.33 g 0.326 2.22
MgCl2 x 6 H2O 0.33 g 0.326 1.606
HEPES 2.38 g 2.354 9.878
Resazurin 1.00 mg 9.9e-4 0.004
Trace minerals 10.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Sulfur
(powder) 		12.00 g 11.869 370.11
Distilled water 900.00 ml - -
1 Mix components except sulfur, adjust pH to 6.8 and autoclave under a N2-CO2 (4:1, v/v) atmosphere. Distribute medium into culture vessels (e.g., 18 ml in 100 ml serum bottles) containing appropriate amount of sulfur under a N2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized) to the above described basal medium (900 ml):
Sodium formate
(15%) 		20.00 ml - -
NaHCO3
(*, 5%) 		50.00 ml - -
Trace vitamins (see Medium No. 197)
(*) 		10.00 ml - -
Na2S x 9 H2O
(3%) 		20.00 ml - -
2 Readjust pH of the medium to 6.8, if necessary.

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 24.738 423.304
Sulfur 11.869 370.11
HEPES 2.354 9.878
NaNO3 0.989 11.637
CaCl2 x 2 H2O 0.327 2.227
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain