MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. J754

Download this solution as

CSV JSON PDF

Internal Solution-ID: S4693

Equipment needed: Hungate tubes Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 30.00 g 29.586 506.259
MgCl2 x 6 H2O 6.50 g 6.41 31.531
CaCl2 x 2 H2O 0.50 g 0.493 3.354
KCl 0.33 g 0.325 4.365
NH4Cl 0.25 g 0.247 4.609
K2HPO4 0.14 g 0.138 0.793
MnCl2 x 4 H2O 4.00 mg 0.004 -
Na2SeO3 x 5 H2O 4.00 mg 0.004 0.015
NiCl2 x 6 H2O 2.00 mg 0.002 0.015
CoCl2 x 6 H2O 2.00 mg 0.002 0.015
ZnCl2 0.50 mg 4.9e-4 0.004
Na2MoO4 x 2 H2O 0.40 mg 3.9e-4 0.002
FeCl2 x 4 H2O 0.25 mg 2.5e-4 0.001
Na2WO2 x 2 H2O 0.10 mg 9.9e-5 -
H3BO3 0.10 mg 9.9e-5 0.002
Sulfur 10.00 g 9.862 307.513
Resazurin 0.50 mg 4.9e-4 0.002
Distilled water 1000.00 ml - -
1 Mix components except sulfur and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (filter-sterilized):
Trace vitamins 1.00 ml - -
NaHCO3
(8%) 		12.50 ml - -
2 Separately steam sulfur for 3 hr on each of 3 successive days.
3 To complete the medium, distribute the solution into sterile culture vessels containing appropriate amount of sulfur (e.g., 5 ml solution and 0.05 g sulfur in Hungate tubes) under a H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Then, add Na2S x 9H2O solution from an anaerobic stock (final 0.1 g/L). After inoculation, pressurize the inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v).
J754 MJ MEDIUM FOR DEFFERIBACTER SP. STRAIN NEJINEJI