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Main sol. J776

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Internal Solution-ID: S4723

Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Glutamic acid 2 g 2 13.593
Trisodium citrate x 2 H2O 2 g 2 6.8
KH2PO4 1 g 1 7.348
NH4Cl 1 g 1 18.695
K2SO4 5 g 5 28.693
MgCl2 x 6 H2O 20 g 20 98.375
NaCl 200 g 200 3422.31
Casamino acids
(BD-Difco) 		4 g 4 -
Yeast extract
(BD-Difco) 		2 g 2 -
FeSO4 x 6 H2O
(0.2%) 		2 ml 0.004 0.026
Trace metal solution 2 ml - -
1 For preparation of solid medium, add the components listed above (reduce the amount of NaCl to 150.0 g) to distilled water and bring volume to 800.0 ml. Separately prepare the following solution:
NaCl 50 g 50 855.578
Agar 20 g 20 59.465
Distilled water 200 ml - -
2 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 5.0 with KOH.
3 Combine both solutions after autoclaving.
J776 MH1 MEDIUM FOR SALIARCHAEUM RUBRODURUM