J776
776 .
MH1 MEDIUM FOR SALIARCHAEUM RUBRODURUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J776 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| L-Glutamic acid | 2 | g | 2 | 13.593 | |
| Trisodium citrate x 2 H2O | 2 | g | 2 | 6.8 | |
| KH2PO4 | 1 | g | 1 | 7.348 | |
| NH4Cl | 1 | g | 1 | 18.695 | |
| K2SO4 | 5 | g | 5 | 28.693 | |
| MgCl2 x 6 H2O | 20 | g | 20 | 98.375 | |
| NaCl | 200 | g | 200 | 3422.31 | |
|
Casamino acids
(BD-Difco) |
4 | g | 4 | - | |
|
Yeast extract
(BD-Difco) |
2 | g | 2 | - | |
|
FeSO4 x 6 H2O
(0.2%) |
2 | ml | 0.004 | 0.026 | |
| Trace metal solution | 2 | ml | - | - | |
| 1 For preparation of solid medium, add the components listed above (reduce the amount of NaCl to 150.0 g) to distilled water and bring volume to 800.0 ml. Separately prepare the following solution: | |||||
| NaCl | 50 | g | 50 | 855.578 | |
| Agar | 20 | g | 20 | 59.465 | |
| Distilled water | 200 | ml | - | - | |
| 2 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 5.0 with KOH. | |||||
| 3 Combine both solutions after autoclaving. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| CoCl2 x 6 H2O | 0.30 | g | 0.3 | 2.311 | |
| MnCl2 x 4 H2O | 0.10 | g | 0.1 | 0.505 | |
| ZnCl2 | 0.10 | g | 0.1 | 0.734 | |
| H3BO4 | 0.02 | g | 0.02 | - | |
| Na2MoO4 x 2 H2O | 0.10 | g | 0.1 | 0.413 | |
| NiCl2 x 6 H2O | 0.10 | g | 0.1 | 0.772 | |
| CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 | |
| AlCl3 | 0.04 | g | 0.04 | 0.3 | |
| Na2WO4 x 2 H2O | 0.02 | g | 0.02 | 0.061 | |
| CaCl2 x 2 H2O | 1.00 | g | 1 | 6.802 | |
| BaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.409 | |
| Na2S2O3 x 5 H2O | 2.00 | g | 2 | 8.058 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 4.0 with HCl. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 4.0 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 9 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 50 | 855.578 |
| MgCl2 x 6 H2O | 20 | 98.375 |
| Agar | 20 | 59.465 |
| K2SO4 | 5 | 28.693 |
| Casamino acids | 4 | - |
| Show more | ||
