Main sol. J784
Internal Solution-ID: S4733
Equipment needed: Autoclave
Anaerobic workbench
Hungate tubes
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 0.450 | g | 0.446 | 3.375 | |
| KH2PO4 | 0.050 | g | 0.05 | 0.364 | |
| MgSO4 x 7 H2O | 0.500 | g | 0.496 | 2.011 | |
| KCl | 0.050 | g | 0.05 | 0.665 | |
| Yeast extract | 0.500 | g | 0.496 | - | |
| Ca(NO3)2 x 4 H2O | 0.014 | g | 0.014 | 0.059 | |
| Na2SO4 | 3.000 | g | 2.973 | 20.932 | |
| Trace element solution | 1.000 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.500 | g | 0.496 | - | |
| Resazurin | 1.000 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 900.000 | ml | - | - | |
| 1 Mix components thoroughly, distribute the medium into culture vessels (e.g., 4.5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 900 ml medium) from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
|
Na2HPO4
(5%) |
20.000 | ml | - | - | |
|
KH2PO4
(25%) |
40.000 | ml | - | - | |
|
Fructose
(1 M) |
20.000 | ml | - | - | |
|
Trace vitamins (see Medium No. 197)
(*) |
20.000 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
8.000 | ml | - | - | |
| 2 Check final pH of the medium to be around 5.8. | |||||
Media with this solution
| J784 | DESULFOSPOROSINUS ACIDOPHILUS MEDIUM |