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Solution B

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Internal Solution-ID: S4764

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.30 g 2.632 49.198
MgCl2 x 6 H2O 0.10 g 0.877 4.315
CaCl2 x 2 H2O 0.01 g 0.088 0.597
Distilled water 50.00 ml - -
1 Autoclave solutions A and B under a N2 atmosphere. After cooling, combine Solutions A and B together with the following solutions (filter-sterilized) under a N2-CO2 (4:1, v/v) gas mixture:
Trace element solution 1.00 ml - -
FeCl2 solution 1.00 ml - -
Trace vitamins 10.00 ml - -
NaHCO3
(8%) 		50.00 ml - -
2 Distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solution (autoclaved and stored under a N2 atmosphere):
Na2S x 9 H2O
(5%) 		2.00 ml - -
J814 TEPIDIMICROBIUM XYLANILYTICUM MEDIUM