Main sol. J819
Internal Solution-ID: S4773
Equipment needed: Filter
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 1.20 | g | 1.186 | 20.29 | |
| KCl | 0.30 | g | 0.296 | 3.976 | |
| NH4Cl | 0.30 | g | 0.296 | 5.542 | |
| K2HPO4 | 0.20 | g | 0.198 | 1.135 | |
| Na2SO4 | 0.30 | g | 0.296 | 2.087 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.395 | 1.944 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.148 | 1.008 | |
| NaHCO3 | 0.60 | g | 0.593 | 7.058 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Resazurin | 0.50 | mg | 4.9e-4 | 0.002 | |
| Trace vitamins | 10.00 | ml | - | - | |
| Vitamin solution | 1.00 | ml | - | - | |
| Sodium lactate | 1.10 | g | 1.087 | 9.7 | |
| Yeast extract | 0.80 | g | 0.791 | - | |
| KNO3 | 0.50 | g | 0.494 | 4.887 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the medium anaerobically under 100% N2. Add vitamins (filter-sterilized), sodium lactate and nitrate from sterile, anaerobic stock solutions. Adjust final pH to 7.4 - 7.8. | |||||
Media with this solution
| J819 | BACILLUS MACYAE MEDIUM |