Solution A
Internal Solution-ID: S4782
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.0 | g | 1.109 | 20.726 | |
| KH2PO4 | 0.3 | g | 0.333 | 2.444 | |
| K2HPO4 | 0.3 | g | 0.333 | 1.91 | |
| NaCl | 100.0 | g | 110.865 | 1897.07 | |
| KCl | 4.0 | g | 4.435 | 59.485 | |
| Na2SO4 | 3.0 | g | 3.326 | 23.416 | |
| Sodium acetate | 1.0 | g | 1.109 | 13.515 | |
| Sodium lactate | 1.0 | g | 1.109 | 9.893 | |
| Yeast extract | 1.0 | g | 1.109 | - | |
|
Trypticase peptone
(BD-BBL) |
1.0 | g | 1.109 | - | |
| FeCl2 solution | 1.0 | ml | - | - | |
| Trace element solution | 1.0 | ml | - | - | |
| Na2SeO3 x 5 H2O | 3.0 | �g | - | - | |
| Resazurin | 1.0 | mg | 0.001 | 0.005 | |
| Distilled water | 900.0 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 7.0. Autoclave under a N2 atmosphere. | |||||
Media with this solution
| J824 | DESULFOHALOBIUM MEDIUM |