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Solution B

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Internal Solution-ID: S4783

Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 20.0 g 181.818 894.321
CaCl2 x 2 H2O 2.7 g 24.546 166.959
Distilled water 100.0 ml - -
1 To complete the medium, combine Solutions A and B under a N2 atmosphere. Then, add per liter the following solution from an anaerobic stock:
Na2S x 9 H2O
(3%) 		10.0 ml - -
2 Autoclave under a N2 atmosphere.
J824 DESULFOHALOBIUM MEDIUM