Main sol. J825
Internal Solution-ID: S4784
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Tryptone
(BD-Difco) |
1.00 | g | 0.979 | 13.779 | |
Peptone | 1.00 | g | 0.979 | - | |
Yeast extract | 1.00 | g | 0.979 | - | |
K2HPO4 | 1.60 | g | 1.567 | 8.997 | |
NaH2PO4 x 2 H2O | 1.00 | g | 0.979 | 8.164 | |
NH4Cl | 0.50 | g | 0.49 | 9.155 | |
MgSO4 x 7 H2O | 0.16 | g | 0.157 | 0.636 | |
CaCl2 x 2 H2O | 0.06 | g | 0.059 | 0.4 | |
Trace element solution SL-4 | 10.00 | ml | - | - | |
Trace vitamins | 10.00 | ml | - | - | |
Resazurin | 0.50 | mg | 4.9e-4 | 0.002 | |
Distilled water | 930.00 | ml | - | - | |
1 Mix components thoroughly and adjust pH to 7.0. Autoclave the medium under a N2 atmosphere. Add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized): | |||||
Glucose
(10%) |
50.00 | ml | - | - | |
NaHCO3
(*, 8%) |
1.25 | ml | - | - | |
L-Cysteine HCl x H2O
(3%) |
10.00 | ml | - | - | |
Na2S x 9 H2O
(3%) |
10.00 | ml | - | - |
J825 | KOKO MEDIUM |