Main sol. J868
Internal Solution-ID: S4854
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.3 | g | 0.298 | 2.189 | |
| K2HPO4 | 0.3 | g | 0.298 | 1.71 | |
| NH4Cl | 1.0 | g | 0.993 | 18.565 | |
| NaCl | 1.0 | g | 0.993 | 16.993 | |
| KCl | 0.1 | g | 0.099 | 1.332 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.099 | 0.675 | |
| Na2SO4 | 3.0 | g | 2.979 | 20.974 | |
| Sodium acetate | 1.0 | g | 0.993 | 12.106 | |
| Trace element solution | 1.0 | ml | - | - | |
| Yeast extract | 1.0 | g | 0.993 | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.497 | - | |
| Resazurin | 0.5 | mg | 5.0e-4 | 0.002 | |
| Distilled water | 950.0 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 7.2. Distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 950 ml medium) from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
25.0 | ml | - | - | |
|
Fructose
(1 M) |
20.0 | ml | - | - | |
|
Vitamin solution (see Medium No. 403)
(*) |
1.0 | ml | - | - | |
|
Thiamine solution (see Medium No. 403)
(*) |
1.0 | ml | - | - | |
|
Vitamin B12 solution (see Medium No. 403)
(*) |
1.0 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
8.0 | ml | - | - | |
Media with this solution
| J868 | GEOMONAS SACCHAROVORANS MEDIUM |