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JCM 868 . GEOMONAS SACCHAROVORANS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.3 g 0.298 2.189
K2HPO4 0.3 g 0.298 1.71
NH4Cl 1.0 g 0.993 18.565
NaCl 1.0 g 0.993 16.993
KCl 0.1 g 0.099 1.332
CaCl2 x 2 H2O 0.1 g 0.099 0.675
Na2SO4 3.0 g 2.979 20.974
Sodium acetate 1.0 g 0.993 12.106
Trace element solution 1.0 ml - -
Yeast extract 1.0 g 0.993 -
L-Cysteine HCl x H2O 0.5 g 0.497 -
Resazurin 0.5 mg 5.0e-4 0.002
Distilled water 950.0 ml - -
1 Mix components thoroughly and adjust pH to 7.2. Distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 950 ml medium) from anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO3
(*, 8%)
25.0 ml - -
Fructose
(1 M)
20.0 ml - -
Vitamin solution (see Medium No. 403)
(*)
1.0 ml - -
Thiamine solution (see Medium No. 403)
(*)
1.0 ml - -
Vitamin B12 solution (see Medium No. 403)
(*)
1.0 ml - -
Na2S x 9 H2O
(5%)
8.0 ml - -
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2SO4 2.979 20.974
NH4Cl 0.993 18.565
NaCl 0.993 16.993
Sodium acetate 0.993 12.106
Yeast extract 0.993 -
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