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J439. DESULFOVIBRIO AEROTOLERANS DVO5 MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 0.955 16.343
2 0.40 g 0.382 1.879
3 0.10 g 0.096 0.65
4 0.25 g 0.239 4.464
5 2.00 g 1.91 14.037
6 0.50 g 0.478 6.406
7 0.50 g 0.478 -
8 4.00 g 3.82 26.896
9 Trace element solution 1.00 ml - -
10 Selenite-tungstate solution 1.00 ml - -
11 1.00 mg 9.6e-4 0.004
12 1000.00 ml 953.397 52921.5
13
(1.0 M)
20.00 ml - -
14 Vitamin solution
(*)
1.00 ml - -
15 Thiamine solution
(*)
1.00 ml - -
16 Vitamin B12 solution
(*)
1.00 ml - -
17
(*, 8.0%)
20.00 ml 3.299 39.275
18
(5%)
2.00 ml - -
Instructions:
  1. Mix components thoroughly and adjust pH to 7.0. Bring to a boil, and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels (e.g., 10 ml medium in Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, anaerobically and aseptically add per liter the following solutions (autoclaved or *filter-sterilized): #13-17
  2. Prior to use, add per liter the following solution (autoclaved and stored under a N2 atmosphere): #18
Solution occurs in 38 other media (first defined in medium J439 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(25%)
12.5 ml 3.963 108.679
2 2.1 g 2.1 7.553
3 30.0 mg 0.03 0.485
4 100.0 mg 0.1 -
5 190.0 mg 0.19 -
6 24.0 mg 0.024 -
7 2.0 mg 0.002 -
8 144.0 mg 0.144 -
9 36.0 mg 0.036 0.149
10 987.0 ml 985.23 54688.5
Solution occurs in 14 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4 mg 0.04 0.292
2 1 mg 0.01 0.041
3 10 mg 0.1 0.812
4 5 mg 0.05 -
5 10 mg 0.1 -
6
(10 mM, pH 7.1)
100 ml - -
Solution occurs in 69 other media (first defined in medium J431 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 0.4 10.001
2 6.0 mg 0.006 -
3 8.0 mg 0.008 -
4 1000.0 ml 998.207 55408.8
Solution occurs in 10 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10 mg 0.1 -
2
(25 mM, pH 3.4)
100 ml - -
Solution occurs in 18 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 mg 0.05 0.032
2 100 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 956.245 53079.6
Na2SO4 3.82 26.896
NaHCO3 3.299 39.275
KH2PO4 1.91 14.037
NaCl 0.955 16.343
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