JCM 439 . DESULFOVIBRIO AEROTOLERANS DVO5 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.00 g 0.955 16.343
MgCl2 x 6 H2O 0.40 g 0.382 1.879
CaCl2 x 2 H2O 0.10 g 0.096 0.65
NH4Cl 0.25 g 0.239 4.464
KH2PO4 2.00 g 1.91 14.037
KCl 0.50 g 0.478 6.406
Yeast extract 0.50 g 0.478 -
Na2SO4 4.00 g 3.82 26.897
Trace element solution 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Resazurin 1.00 mg 9.6e-4 0.004
Distilled water 1000.00 ml - -
1 Mix components thoroughly and adjust pH to 7.0. Bring to a boil, and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels (e.g., 10 ml medium in Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, anaerobically and aseptically add per liter the following solutions (autoclaved or *filter-sterilized):
Sodium lactate
(1.0 M)
20.00 ml - -
Vitamin solution
(*)
1.00 ml - -
Thiamine solution
(*)
1.00 ml - -
Vitamin B12 solution 0.05 g/L
(*)
1.00 ml - -
NaHCO3
(*, 8.0%)
20.00 ml - -
2 Prior to use, add per liter the following solution (autoclaved and stored under a N2 atmosphere):
Na2S x 9 H2O
(5%)
2.00 ml - -
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -
Solution occurs in 14 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
p-Aminobenzoic acid 4 mg 0.04 0.292
Biotin 1 mg 0.01 0.041
Nicotinic acid 10 mg 0.1 0.812
DL-Calcium pantothenate 5 mg 0.05 0.105
Pyridoxine hydrochloride 10 mg 0.1 -
Sodium phosphate buffer
(10 mM, pH 7.1)
100 ml - -
Solution occurs in 10 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine HCl 10 mg 0.1 -
Sodium phosphate buffer
(25 mM, pH 3.4)
100 ml - -
Solution occurs in 18 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 5 mg 0.05 0.037
Distilled water 100 ml - -

Metadata

Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2SO4 3.82 26.897
KH2PO4 1.91 14.037
NaCl 0.955 16.343
KCl 0.478 6.406
Yeast extract 0.478 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain