Main sol. J874
Internal Solution-ID: S4861
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.1 | g | 0.097 | 0.715 | |
| K2HPO4 | 0.1 | g | 0.097 | 0.558 | |
| NH4Cl | 1.0 | g | 0.973 | 18.186 | |
| NaCl | 70.0 | g | 68.093 | 1165.18 | |
| KCl | 0.1 | g | 0.097 | 1.305 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.097 | 0.662 | |
| Trace element solution | 10.0 | ml | - | - | |
| Yeast extract | 1.0 | g | 0.973 | - | |
| Na2S2O3 x 5 H2O | 5.0 | g | 4.864 | 19.598 | |
| Resazurin | 1.0 | mg | 9.7e-4 | 0.004 | |
| Distilled water | 935.0 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 7.5 with KOH and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (autoclaved and stored under N2, or *filter-sterilized and stored under N2-CO2, 4:1, v/v): | |||||
|
Glucose
(1 M) |
20.0 | ml | - | - | |
|
NaHCO3
(*, 8%) |
25.0 | ml | - | - | |
|
MgCl2 x 6 H2O
(15%) |
20.0 | ml | - | - | |
| 2 Aseptically distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solutions (autoclaved and stored under N2): | |||||
|
L-Cysteine HCl x H2O
(5%) |
10.0 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
8.0 | ml | - | - | |
Media with this solution
| J874 | BELH1 MEDIUM |