Main sol. J899
Internal Solution-ID: S4894
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.20 | g | 0.198 | 1.458 | |
| NH4Cl | 0.25 | g | 0.248 | 4.637 | |
| NaCl | 1.00 | g | 0.992 | 16.976 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.397 | 1.952 | |
| KCl | 0.50 | g | 0.496 | 6.654 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.099 | 0.675 | |
| NaHCO3 | 0.10 | g | 0.099 | 1.181 | |
| Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
|
Yeast extract
(BD-Difco) |
2.00 | g | 1.984 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly and autoclave under a N2 gas atmosphere. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
|
Vitamin solution
(*) |
1.00 | ml | - | - | |
|
Trace element solution
(*) |
1.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
6.00 | ml | - | - | |
| 2 Check pH to be 7.5 - 7.6. | |||||
Media with this solution
| J899 | THERMOBACTER ROSEUM MEDIUM |