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Main sol. J940

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Internal Solution-ID: S4952

Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 0.192 1.412
NH4Cl 0.25 g 0.24 4.49
NaCl 1.00 g 0.961 16.438
KCl 0.50 g 0.48 6.443
MgCl2 x 6 H2O 0.40 g 0.384 1.89
CaCl2 x 2 H2O 0.15 g 0.144 0.98
Sodium pyruvate 1.10 g 1.057 9.603
Yeast extract 2.00 g 1.921 -
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Resazurin 0.40 mg 3.8e-4 0.002
Distilled water 1000.00 ml - -
1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (filter-sterilized):
NaHCO3
(8%) 		30.00 ml - -
Vitamin solution 1.00 ml - -
2 Distribute the medium into culture vessels under the same gas mixture. Finally, add per liter the following solution from an anaerobic stock:
Na2S x 9 H2O
(5%) 		7.00 ml - -
J940 AMINIVIBRIO ASINOENSIS MEDIUM