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Trace elements solution

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Internal Solution-ID: S4963

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
Fe(NH4)2(SO4)2 x 6 H2O 0.20 g 0.2 -
Na2SeO3 x 5 H2O 0.20 g 0.2 0.76
MnCl2 x 4 H2O 0.11 g 0.11 -
CoCl2 x 6 H2O 0.10 g 0.1 0.77
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
Na2WO4 x 2 H2O 0.10 g 0.1 -
NiCl2 x 6 H2O 0.25 g 0.25 1.929
ZnSO4 x 7 H2O 0.20 g 0.2 0.696
H3BO3 0.01 g 0.01 0.162
CuSO4 x 5 H2O 2.00 mg 0.002 0.008
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.
J944 MW MEDIUM FOR METHANOSARCINA