Main sol. J989
Internal Solution-ID: S5019
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.0 | g | 0.998 | 18.658 | |
| KH2PO4 | 0.3 | g | 0.299 | 2.2 | |
| K2HPO4 | 0.3 | g | 0.299 | 1.719 | |
| NaCl | 20.0 | g | 19.96 | 341.548 | |
| KCl | 0.1 | g | 0.1 | 1.339 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.679 | |
| L-Serine | 1.0 | g | 0.998 | 9.497 | |
|
Yeast extract
(BD-Difco) |
2.0 | g | 1.996 | - | |
| Trace element solution | 1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.499 | - | |
| Resazurin | 0.5 | mg | 5.0e-4 | 0.002 | |
| Distilled water | 945.0 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 7.2. Bring to a boil, cool down under a N2-CO2 (4:1, v/v) gas stream, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add the following stock solutions [autoclaved and stored under a N2 atmosphere or *filter-sterilize and store under a N2-CO2 (4:1, v/v) gas mixture]: | |||||
|
MgCl2 x 6 H2O
(15%) |
20.0 | ml | - | - | |
|
NaHCO3
(*, 8%) |
25.0 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.0 | ml | - | - | |
| 2 Readjust pH to 7.2, if necessary. | |||||
Media with this solution
| J989 | IRD AMINOBACTERIUM MEDIUM |