MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. J1009

Download this solution as

CSV JSON PDF

Internal Solution-ID: S5050

Equipment needed: Anaerobic workbench Hungate tubes Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.311 5.809
KCl 0.33 g 0.311 4.168
KH2PO4 0.33 g 0.311 2.283
Yeast extract
(BD-Difco) 		0.50 g 0.471 -
Tryptone
(BD-Difco) 		3.00 g 2.825 39.742
Trace element solution SL-10 1.00 ml - -
Trace vitamins 10.00 ml - -
CaCl2 x 2 H2O
(1%) 		33.00 ml - -
MgCl2 x 6 H2O
(2%) 		16.50 ml - -
L-Cysteine HCl x H2O 0.50 g 0.471 -
Resazurin
(1%) 		1.00 ml - -
Distilled water 1000.00 ml - -
1 Prepare the medium anaerobically under a N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 5.0 ml in Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After autoclaving, add per 5.0 ml the following solutions from anaerobic stocks (autoclaved or *filter-sterilized and stored under a N2 atmosphere):
Na2S x 9 H2O
(w/v, 3%) 		0.05 ml - -
NaHCO3
(w/v, *, 10%) 		0.05 ml - -
Glucose
(w/v, 20%) 		0.10 ml - -
2 Adjust pH to 7.3.
J1009 CLOSTRIDIUM SWELLFUNIANUM MEDIUM