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Main sol. J1027

Internal Solution-ID: S5078

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NH₄Cl	0.54	g	0.523	9.773
KH₂PO₄	0.14	g	0.136	0.996
MgCl₂ x 6 H₂O	3.50	g	3.388	16.666
K₂HPO₄	0.30	g	0.29	1.667
CaCl₂ x 2 H₂O	0.15	g	0.145	0.988
NaCl	5.00	g	4.84	82.825
NaHCO₃	2.52	g	2.44	29.039
Trace mineral solution	1.00	ml	-	-
Se/W solution	1.00	ml	-	-
Trace vitamins solution	1.00	ml	-	-
Resazurin	1.00	mg	9.7e-4	0.004
Distilled water	1000.00	ml	-	-
1 Mix components thoroughly and distribute the medium into culture vessels (e.g., 20 ml medium in 100 ml serum bottles or 5.0 ml medium in Hungate tubes) under a stream of H₂-CO₂ (80:20, v/v), seal with butyl rubber stoppers and autoclave. Allow to stand the autoclaved medium overnight. Add per liter the following solution from a sterile anaerobic stock (filter-sterilized and stored under a N₂ atmosphere):
Trimethylamine (1 M)	10.00	ml	-	-
2 Prior to inoculation, add per liter the following solutions aseptically and anaerobically (autoclaved and stored under a N₂ atmosphere):
L-Cysteine HCl x H₂O (5%)	10.00	ml	-	-
Na₂S x 9 H₂O (5%)	10.00	ml	-	-
3 Comments: For strain JCM 19935, replace trimethylamine with yeast extract (final 0.1 g/L) and pressurize to 100 kPa H₂-CO₂ (80:20, v/v). For strain JCM 19936, prepare the medium without trimethylamine. Pressurize inoculated culture vessels to 100 kPa H₂-CO₂ (80:20, v/v).

Compound

Amount

Unit

Conc. [g/L]

Conc. [mM]

NH₄Cl

0.54

0.523

9.773

KH₂PO₄

0.14

0.136

0.996

MgCl₂ x 6 H₂O

3.50

3.388

16.666

K₂HPO₄

0.30

0.29

1.667

CaCl₂ x 2 H₂O

0.15

0.145

0.988

NaCl

5.00

4.84

82.825

NaHCO₃

2.52

2.44

29.039

Trace mineral solution

1.00

Se/W solution

1.00

Trace vitamins solution

1.00

Resazurin

1.00

9.7e-4

0.004

Distilled water

1000.00

1 Mix components thoroughly and distribute the medium into culture vessels (e.g., 20 ml medium in 100 ml serum bottles or 5.0 ml medium in Hungate tubes) under a stream of H₂-CO₂ (80:20, v/v), seal with butyl rubber stoppers and autoclave. Allow to stand the autoclaved medium overnight. Add per liter the following solution from a sterile anaerobic stock (filter-sterilized and stored under a N₂ atmosphere):

Trimethylamine
(1 M)

10.00

2 Prior to inoculation, add per liter the following solutions aseptically and anaerobically (autoclaved and stored under a N₂ atmosphere):

L-Cysteine HCl x H₂O
(5%)

10.00

Na₂S x 9 H₂O
(5%)

10.00

3 Comments: For strain JCM 19935, replace trimethylamine with yeast extract (final 0.1 g/L) and pressurize to 100 kPa H₂-CO₂ (80:20, v/v). For strain JCM 19936, prepare the medium without trimethylamine. Pressurize inoculated culture vessels to 100 kPa H₂-CO₂ (80:20, v/v).

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Main sol. J1027

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Main sol. J1027

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