Main sol. J1051
Internal Solution-ID: S5112
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 1.0 | g | 0.996 | 17.043 | |
| 1 Adjust pH to 7.1 - 7.2. Distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solution (filter-sterilized): | |||||
| KCl | 0.5 | g | 0.498 | 6.68 | |
| MgCl2 x 6 H2O | 0.4 | g | 0.398 | 1.96 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.677 | |
| NH4Cl | 0.3 | g | 0.299 | 5.586 | |
| KH2PO4 | 0.2 | g | 0.199 | 1.464 | |
| Resazurin | 0.4 | mg | 4.0e-4 | 0.002 | |
| Distilled water | 945.0 | ml | - | - | |
| 2 Mix components thoroughly and autoclave. Cool medium under a N2-CO2 (4:1, v/v) gas stream, and add the following solutions (autoclaved or *filter-sterilized) anaerobically and aseptically: | |||||
|
NaHCO3
(*, 8%) |
30.0 | ml | - | - | |
|
Glucose
(*, 1 M) |
5.0 | ml | - | - | |
|
L-Cysteine HCl x H2O
(0.1 M) |
10.0 | ml | - | - | |
|
Yeast extract
(*, 10%) |
2.0 | ml | - | - | |
|
Casamino acids
(*, 10%) |
2.0 | ml | - | - | |
| Trace element solution | 1.0 | ml | - | - | |
| FeCl2 solution | 1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
|
Trace vitamins solution (see Medium No. 284)
(*) |
1.0 | ml | - | - | |
| Fatty acid mixture | 1.0 | ml | - | - | |
| Aromatic fatty acids solution | 1.0 | ml | - | - | |
|
Dithiothreitol
(250 mM) |
4.0 | ml | - | - | |
Media with this solution
| J1051 | ENDOMICROBIUM PROAVITUM RSA MEDIUM |