Main sol. J1053
Internal Solution-ID: S5115
Equipment needed: Anaerobic workbench
Filter
Autoclave
| 1 Mix components thoroughly, adjust pH to 6.0 with 1 N HCl and autoclave under a H2-CO2 (4:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 15.00 | g | 14.97 | 256.161 | |
| MgCl2 x 6 H2O | 4.00 | g | 3.992 | 19.636 | |
| KCl | 0.33 | g | 0.329 | 4.418 | |
| CaCl2 x 2 H2O | 0.33 | g | 0.329 | 2.24 | |
| (NH4)2SO4 | 0.50 | g | 0.499 | 3.776 | |
| FeCl2 solution | 1.00 | ml | - | - | |
| Trace element solution | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 0.50 | ml | - | - | |
| Distilled water | 860.00 | ml | - | - | |
|
KH2PO4
(3.3%) |
10.00 | ml | - | - | |
|
Trace vitamins
(*) |
10.00 | ml | - | - | |
|
NaHCO3
(*, 8%) |
10.00 | ml | - | - | |
|
(w/v) MES (pH 6.0, 20%) |
10.00 | ml | - | - | |
| Iron(III) citrate solution | 100.00 | ml | - | - | |
| 2 Readjust pH to 6.0 to 6.3, if necessary. | |||||
Media with this solution
| J1053 | DEFERRISOMA PALEOCHRORII MEDIUM |