Main sol. J1073
Internal Solution-ID: S5138
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 3.00 | g | 2.947 | 20.747 | |
| KH2PO4 | 0.20 | g | 0.196 | 1.444 | |
| NH4Cl | 0.30 | g | 0.295 | 5.509 | |
| NaCl | 13.50 | g | 13.261 | 226.922 | |
| MgCl2 x 6 H2O | 2.20 | g | 2.161 | 10.63 | |
| KCl | 0.50 | g | 0.491 | 6.588 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.147 | 1.002 | |
| Na2SeO3 | 3.00 | μg | - | - | |
| FeCl2 solution | 1.00 | ml | - | - | |
| Trace element solution | 1.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
| Yeast extract | 0.50 | g | 0.491 | - | |
| Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 950.00 | ml | - | - | |
| 1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
30.00 | ml | - | - | |
|
L-Sodium lactate
(10%) |
20.00 | ml | - | - | |
| 2 Aseptically distribute the medium into culture vessels under and a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution (autoclaved and stored under a N2 atmosphere): | |||||
|
Na2S x 9 H2O
(5%) |
6.00 | ml | - | - | |
Media with this solution
| J1073 | MARINE SRB MEDIUM |