Soda based mineral medium
Internal Solution-ID: S5149
This solution is used in 3 media and was first defined in medium J1081.
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Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2CO3 | 185 | g | 46250 | 436370 | |
| NaHCO3 | 35 | g | 8750 | 104158 | |
| NaCl | 16 | g | 4000 | 68446.3 | |
| K2HPO4 | 1 | g | 250 | 1435.33 | |
| KCl | 5 | g | 1250 | 16767.3 | |
| 1 Add components to distilled water and bring volume to 1.0 L. Autoclave and let it stand for three days. Decant the clear solution into another empty sterile bottle. Aseptically add per liter the following solutions (autoclaved): | |||||
|
NH4Cl
(4 M) |
1 | ml | - | - | |
| Selenite-tungstate solution | 1 | ml | - | - | |
| Trace element solution | 1 | ml | - | - | |
|
MgCl2
(1 M) |
1 | ml | - | - | |