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JCM 1081 . NATRANAEROARCHAEUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1081 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Basal mineral NaCl medium
(sterilized, see Medium No. 1079) 		250.0 ml - -
Soda based mineral medium
(sterilized) 		750.0 ml - -
1 To complete the medium, aseptically add the following solution (autoclaved or *filter-sterilized):
Sodium pyruvate
(*, 2 M) 		5.0 ml - -
Trace vitamins (see Medium No. 197)
(*) 		10.0 ml - -
Yeast extract
(10%) 		0.2 ml - -

Soda based mineral medium #

4
Solution occurs in 2 other media (first defined in Medium J1081).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 185 g 46250 436370
NaHCO3 35 g 8750 104158
NaCl 16 g 4000 68446.3
K2HPO4 1 g 250 1435.33
KCl 5 g 1250 16767.3
1 Add components to distilled water and bring volume to 1.0 L. Autoclave and let it stand for three days. Decant the clear solution into another empty sterile bottle. Aseptically add per liter the following solutions (autoclaved):
NH4Cl
(4 M) 		1 ml - -
Selenite-tungstate solution 1 ml - -
Trace element solution 1 ml - -
MgCl2
(1 M) 		1 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Trace element solution #

1000
Solution occurs in 13 other media (first defined in Medium J1079).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 -
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 Adjust pH to 3.6.

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 34174.9 322441
NaHCO3 6465.52 76964
NaCl 2955.67 50576.1
KCl 923.645 12389.6
K2HPO4 184.729 1060.59
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Cultivation metadata from 1 strains BacDive

pH 8 - 10 1 strain
pH 10 - 12 1 strain
phyla 1 1 strain
oxygen facultative anaerobe 1 strain
temperature mesophilic 1 strain
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