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Main sol. J1090

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Internal Solution-ID: S5161

Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 19.608 335.521
K2HPO4 0.14 g 0.137 0.788
CaCl2 x 2 H2O 1.00 g 0.98 6.669
MgSO4 x 7 H2O 3.40 g 3.333 13.524
MgCl2 x 6 H2O 4.18 g 4.098 20.157
NH4Cl 0.25 g 0.245 4.582
KCl 0.33 g 0.324 4.34
NiCl2 x 6 H2O 0.50 mg 4.9e-4 0.004
Na2SeO3 x 5 H2O 0.50 mg 4.9e-4 0.002
Na2WO4 x 2 H2O 0.10 mg 9.8e-5 3.0e-4
(NH4)2Ni(SO4)2 x 6 H2O 10.00 mg 0.01 0.025
Trace minerals 10.00 ml - -
Distilled water 1000.00 ml - -
1 Mix components and adjust pH to 5.5. Distribute the medium into culture vessels (e.g., 20 ml in 60 ml-serum bottles) under a stream of N2, seal with butyl rubber stoppers and add Autoclave. After cooling, add per liter the following solutions (filter-sterilized):
Trace vitamins 1.00 ml - -
NaHCO3
(8%) 		3.75 ml - -
Na2S2O3
(1 M) 		5.00 ml - -
2 Replace the gas phase with N2-CO2-O2 (94:5:1, v/v/v, 100 kPa). Check final pH to be around 6.2.
J1090 MMJS MEDIUM 2