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Main sol. J1095

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Internal Solution-ID: S5166

Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 0.53 g 0.523 3.682
KH2PO4 0.41 g 0.404 2.971
NH4Cl 0.30 g 0.296 5.531
NaCl 0.30 g 0.296 5.063
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Selenite-tungstate solution 0.50 ml - -
Yeast extract 0.10 g 0.099 -
Resazurin 0.50 mg 4.9e-4 0.002
Distilled water 930.00 ml - -
1 Mix components and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (autoclaved or *filter-sterilized):
MgCl2 x 6 H2O
(10%) 		1.00 ml - -
CaCl2 x 2 H2O
(10%) 		1.00 ml - -
Trace vitamins
(*) 		10.00 ml - -
NaHCO3
(*, 8%) 		50.00 ml - -
Substrate Solution 10.00 ml - -
2 Aseptically and anaerobically distribute the medium into culture vessels (e.g., 50 ml of the medium in 120 ml serum bottle) under a N2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Prior to use, add per liter the following solution (autoclaved and stored under a N2 atmosphere):
Na2S x 9 H2O
(5%) 		10.00 ml - -
J1095 BICARBONATE BUFFERED MEDIUM