Main sol. J1104
Internal Solution-ID: S5192
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 0.02 | g | 0.02 | 0.14 | |
| KH2PO4 | 1.00 | g | 0.997 | 7.326 | |
| NH4Cl | 0.10 | g | 0.1 | 1.864 | |
| NaCl | 20.00 | g | 19.94 | 341.208 | |
| MgCl2 x 6 H2O | 3.00 | g | 2.991 | 14.712 | |
| KCl | 0.50 | g | 0.499 | 6.687 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.017 | |
| FeCl2 solution | 1.00 | ml | - | - | |
| Trace element solution | 1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 950.00 | ml | - | - | |
| 1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) atmosphere. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
30.00 | ml | - | - | |
|
Vitamin solution
(*) |
1.00 | ml | - | - | |
|
Yeast extract
(10%) |
5.00 | ml | - | - | |
|
Maltose
(1 M) |
5.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
| 2 Readjust pH to 6.8 - 7.2, if necessary. | |||||
Media with this solution
| J1104 | HYPNOCYCLICUS MEDIUM |