Main sol. J1106
Internal Solution-ID: S5194
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 22.00 | g | 22 | 376.454 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.14 | 0.952 | |
| KCl | 0.34 | g | 0.34 | 4.561 | |
| NH4Cl | 0.50 | g | 0.5 | 9.348 | |
| MgCl2 x 6 H2O | 2.75 | g | 2.75 | 13.527 | |
| K2HPO4 | 0.14 | g | 0.14 | 0.804 | |
|
Yeast extract
(BD-Difco) |
0.20 | g | 0.2 | - | |
| Trace mineral solution | 10.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 930.00 | ml | - | - | |
| 1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
30.00 | ml | - | - | |
|
Trace vitamins
(*) |
10.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
|
L-Cysteine HCl x H2O
(5%) |
10.00 | ml | - | - | |
Media with this solution
| J1106 | MARINE METHANOGENIC MEDIUM |