Main sol. J1116
Internal Solution-ID: S5206
Equipment needed: Filter
Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.30 | g | 0.303 | 5.671 | |
| KH2PO4 | 0.20 | g | 0.202 | 1.486 | |
| NaCl | 30.00 | g | 30.334 | 519.057 | |
| KCl | 0.50 | g | 0.506 | 6.782 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.152 | 1.032 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.404 | 1.989 | |
| Na2SO4 | 2.80 | g | 2.831 | 19.932 | |
| Yeast extract | 0.50 | g | 0.506 | - | |
| Trace element solution | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.506 | - | |
| Resazurin | 1.00 | mg | 0.001 | 0.004 | |
| Distilled water | 920.00 | ml | - | - | |
| 1 Mix components and adjust pH to 7.0. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized): | |||||
|
Sodium lactate
(1 M) |
20.00 | ml | - | - | |
|
NaHCO3
(*, 5%) |
40.00 | ml | - | - | |
| 2 Prior to use, add per liter the following solution to reduce the medium (autoclaved and stored under N2): | |||||
|
Na2S x 9 H2O
(5%) |
8.00 | ml | - | - | |
Media with this solution
| J1116 | IRD MARINE DESULFOVIBRIO MEDIUM-2 |