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Main sol. J1136

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Internal Solution-ID: S5236

Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.30 g 0.297 5.559
KH2PO4 0.20 g 0.198 1.457
KCl 0.50 g 0.496 6.647
CaCl2 x 2 H2O 0.15 g 0.149 1.011
MgCl2 x 6 H2O 0.40 g 0.396 1.95
Yeast extract 0.50 g 0.496 -
Trace element solution 1.00 ml - -
L-Cysteine HCl x H2O 0.50 g 0.496 -
Resazurin 1.00 mg 9.9e-4 0.004
Distilled water 960.00 ml - -
1 Mix components and adjust pH to 7.0. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
Glucose
(1 M) 		20.00 ml - -
NaHCO3
(*, 5%) 		20.00 ml - -
2 Prior to use, add per liter the following solution to reduce the medium (autoclaved and stored under N2):
Na2S x 9 H2O
(5%) 		8.00 ml - -
J1136 IRD F1F22 MEDIUM