Main sol. J1149
Internal Solution-ID: S5258
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.50 | g | 0.495 | 3.638 | |
| MgSO4 x 7 H2O | 0.40 | g | 0.396 | 1.607 | |
| NaCl | 5.00 | g | 4.951 | 84.711 | |
| NH4Cl | 1.00 | g | 0.99 | 18.51 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.337 | |
| Sodium acetate x 3 H2O | 2.70 | g | 2.673 | - | |
| Trace element solution | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.495 | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 980.00 | ml | - | - | |
| 1 Mix components and adjust pH to 7.0. Bring to a boil and cool down under a H2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
20.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
8.00 | ml | - | - | |
Media with this solution
| J1149 | METHANOMASSILIICOCCUS ALVUS MEDIUM |