MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. J1151

Download this solution as

CSV JSON PDF

Internal Solution-ID: S5260

Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.00 g 0.973 16.646
KCl 0.50 g 0.486 6.524
MgCl2 x 6 H2O 0.40 g 0.389 1.914
KH2PO4 0.20 g 0.195 1.43
NH4Cl 0.25 g 0.243 4.546
CaCl2 x 2 H2O 0.15 g 0.146 0.993
Na2SO4 1.42 g 1.381 9.725
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Resazurin 0.40 mg 3.9e-4 0.002
Distilled water 970.00 ml - -
1 Mix components and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (filter-sterilized):
Vitamin solution 1.00 ml - -
NaHCO3
(8%) 		30.00 ml - -
2 Aseptically and anaerobically distribute the medium into culture vessels (e.g., 50 ml of the medium in 120 ml serum bottle) under a N2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Prior to use, add per liter the following solution (autoclaved and stored under a N2 atmosphere):
Naphthalene solution
(1.5%) 		20.00 ml - -
Na2S x 9 H2O
(5%) 		4.00 ml - -
J1151 DESULFOBACTERIUM NAPHTHALENIVOLANS MEDIUM