Main sol. J1177
Internal Solution-ID: S5288
Equipment needed: Filter
Autoclave
Anaerobic workbench
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 120.0 | g | 116.505 | 1993.58 | |
| MgCl2 x 6 H2O | 6.0 | g | 5.825 | 28.653 | |
| KCl | 1.5 | g | 1.456 | 19.535 | |
| Na2SO4 | 1.0 | g | 0.971 | 6.835 | |
| NH4Cl | 1.0 | g | 0.971 | 18.151 | |
| CaCl2 x 2 H2O | 0.4 | g | 0.388 | 2.642 | |
| K2HPO4 | 0.4 | g | 0.388 | 2.23 | |
| Trace mineral solution | 10.0 | ml | - | - | |
| Resazurin | 0.5 | mg | 4.9e-4 | 0.002 | |
| Distilled water | 950.0 | ml | - | - | |
| 1 Mix components thoroughly, bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream and autoclave under the same gas mixture. After cooling, add per liter the following solutions (autoclaved or *filter-sterilized): | |||||
|
Glucose
(10%) |
10.0 | ml | - | - | |
|
Trace vitamins
(*) |
10.0 | ml | - | - | |
|
NaHCO3
(*, 8%) |
30.0 | ml | - | - | |
| 2 Aseptically and anaerobically, dispense the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solutions (autoclaved and stored under a N2 atmosphere): | |||||
|
L-Cysteine HCl x H2O
(5%) |
10.0 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.0 | ml | - | - | |
| 3 Readjust pH to 7.5, if necessary. | |||||
Media with this solution
| J1177 | SEDIMENTISPHAERA L21 MEDIUM |