Main sol. J1247
Internal Solution-ID: S5383
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(BD-Difco) |
0.50 | g | 0.5 | - | |
|
Proteose peptone no. 3
(BD-Difco) |
0.50 | g | 0.5 | - | |
|
Casamino acids
(BD-Difco) |
0.50 | g | 0.5 | - | |
| Glucose | 0.50 | g | 0.5 | 2.775 | |
|
Starch
(soluble) |
0.50 | g | 0.5 | - | |
| Sodium pyruvate | 0.30 | g | 0.3 | 2.726 | |
| K2HPO4 | 0.30 | g | 0.3 | 1.722 | |
| MgSO4 x 7 H2O | 0.05 | g | 0.05 | 0.203 | |
|
Sea water
(see below, 30%) |
833.00 | ml | - | - | |
| Distilled water | 167.00 | ml | - | - | |
| 1 30% Seawater solution: | |||||
| NaCl | 234.00 | g | 234 | 4004.11 | |
| MgCl2 x 6 H2O | 39.00 | g | 39 | 191.832 | |
| MgSO4 x 7 H2O | 61.00 | g | 61 | 247.489 | |
| KCl | 6.00 | g | 6 | 80.483 | |
| CaCl2 x 2 H2O | 1.30 | g | 1.3 | 8.843 | |
| NaBr | 0.70 | g | 0.7 | 6.803 | |
| NaHCO3 | 0.20 | g | 0.2 | 2.381 | |
| 2 Adjust pH to 7.5. | |||||
| 3 Add components to distilled water and bring volume to 1.0 L. | |||||
Media with this solution
| J1247 | R2A MEDIUM WITH 25% SALT |