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Main sol. J1255

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Internal Solution-ID: S5392

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 3.000 g 2.941 50.328
KCl 0.150 g 0.147 1.973
Na2SO4 0.300 g 0.294 2.071
MgSO4 x 7 H2O 0.123 g 0.121 0.489
CaCl2 x 2 H2O 0.015 g 0.015 0.1
NH4Cl 0.107 g 0.105 1.961
NaH2PO4 1.380 g 1.353 11.277
Mineral solution 5.000 ml - -
Distilled water 1000.000 ml - -
Yeast extract
(w/v, 10%) 		10.000 ml - -
Sodium Thiosulfate
(0.2 M) 		5.000 ml - -
1 Mix components thoroughly and adjust pH to 6.75 with NaOH. Distribute the medium into culture vessels, leaving &; 80% volume of gas phase (e.g., 5.0 ml in 25 ml serum bottles or Balch tubes), under a stream of N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically add per liter the following solutions (autoclaved):
2 Then, add filter-sterilized H2, CO2 and air to the culture vessels to make the gaseous composition in the gas phase 75% N2, 17% H2, 4% CO2 and 4% air by volume.
J1255 HYDROGENOBACTER FLORENTISSIMUM MEDIUM